CD19 molecule is a glycosylated type I transmembrane protein with a molecular weight of 95KD on the surface of B cells. It is only expressed in B cells of the normal hematopoietic system and follicular dendritic cells (FDC) in the germinal center. Not expressed in cells. The expression of CD19 molecules begins in bone marrow B progenitor cells and continues throughout the maturation phase of B cells until they disappear when they differentiate into plasma cells ^[1] . CD19/CD21/CD81 complex and BCR form a dual antigen binding model of B cells, which participates in the signal transduction of B cells ^[2] ; in addition, CD19 molecules participate in the movement of Ca+ in B cells and regulate the activation and proliferation of B cells; Since CD19 is a marker of B cells, CAR-T targeting CD19 has shown shocking efficacy in the treatment of malignant B lymphocyte tumors ^[3,4] . CAR-T constructed with FMC63 antibody (targeting CD19 murine monoclonal antibody) sequence has become the world's first CAR-T cell drug to be marketed . FMC63 can specifically recognize the extracellular segment of CD19 , so it can be used for flow cytometric detection of CD19 positive cells or for the activity detection of CD19 recombinant protein.

Reactivity: human

Subclass: human IgG1

Concentration: 1μg/μL

Analysis instrument: flow cytometer

Purification method: affinity chromatography

Storage system: PBS, pH 7.4, 20% glycerol

Storage conditions: -20° C refrigerator, avoid repeated freezing and thawing

Illustration: Lane M on the left is protein MW marker, Lane 1 is 2ug CD19 antibody;

The right picture shows the flow cytometric detection of CD19 expression on the surface of CHO-K1 cells

Reference materials:

1. CD19 Is Functionally and Physically Associated With Surface Immunoglobulin

2. CD19 Is Linked to the Integrin-Associated Tetraspans CD9, CD81, and CD82

3. A CD19/Fc Fusion Protein for Detection of anti-CD19 Chimeric Antigen Receptors

4. The Promoter of the CD19 Gene Is a Target for the B-cell-specific Transcription Factor BSAP