CD20, like CD19 , is a specific antigen of B cells, only located in pre- B cells and mature B cells. It is expressed in more than 95 % of B- cell lymphomas, but not in hematopoietic stem cells, plasma cells and other normal tissues. Therefore, it is a popular target for the treatment of lymphoma, leukemia and certain autoimmune diseases, and its application in the field of immunotherapy is very wide. With CD20 as a target of human-mouse chimeric antibody rituximab, it has become a classic antibody drugs in cancer immunotherapy, and with CD20 as a target of CAR-T is also used for multiple clinical trials.

This product is a human-mouse chimeric antibody targeting CD20 (Rituxan antibody sequence), which can be used for flow cytometric detection of over-expressing CD20 cell lines, and can also be used for the activity detection of recombinant CD20 protein.

Product Name: CD20 Recombinant Antibody

Reactivity: human

Subclass: human IgG1

Concentration: 1μg/μL

Analysis instrument: flow cytometer

Purification method: affinity chromatography

Storage system: PBS, pH 7.4, 20% glycerol

Storage conditions: -20°C refrigerator, avoid repeated freezing and thawing

Illustration: Lane M on the left is protein MW marker, Lane 1 is 10ug CD20 antibody;

The right picture shows the FACS detection of the expression of CD20 antibody on the surface of Raji cells

Reference materials:

Induction of cytosolic calcium flux by CD20 is dependent upon B cell antigen receptor signaling