Overexpression stable cell line
Gene overexpression or knockout is one of the commonly used methods to study gene function. Through transient transfection, gene expression peaks are usually observed in the range of 36-48 hours, after which the peak disappears quickly. For sustained gene expression, recombinant stable cell lines must be screened. Lentiviruses can effectively stably integrate foreign genes into the host cell genome, especially for those host cells that are difficult to transfect. Using this feature of lentivirus, it is possible to easily achieve the overexpression of the target gene and the knockdown of the target gene in the target cell, and construct a recombinant cell line that can be passaged stably.
>>Service Features
2 free cells, free storage for 6 months
A variety of promoters and resistance screening markers to meet the needs of different customers
Polyclonal guarantee services, funds without worry
A variety of cloning validation protocols, including molecular level and protein level validation
>>Technical Process
>>Case Show
Lentiviral vector preparation
Lentivirus packaging
Positive cell clone screening
Identification of positive clones
8-10 weeks delivery
Target gene information
Host cell line
Stable cell line
Sequencing report
Q-PCR, Western Blot test results
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