Recombinant human ROR1 protein

【No.】IRP019A 【Protein name】 Recombinant human ROR1 protein
【Gene Sequence Number/Expression Region】Q01973/30-403AA 【Label position】C-terminal Human-Fc Tag
【Restriction sites】Enterokinase 【Packing specification】100μg/tube

ROR1 and ROR2 are orphan receptor tyrosine kinases, which are most closely related to the MuSK and Trk family of neurotrophin receptors. They have an extracellular coil-like cysteine-rich domain and a three-ring domain near the membrane end, both of which are assumed to mediate protein-protein interactions [1-3] . In Caenorhabditis elegans, Drosophila, mice and humans, the ROR family RTK is very conservative in evolution [1,4] . Although the function of ROR kinase is unclear, based on the similarity between ROR and MuSK and Trk kinases, it can be inferred that ROR kinase can regulate the development of synapses. CAM-1 is a homologous gene of ROR family RTK in Caenorhabditis elegans. It plays a variety of important roles in the regulation of cell migration, asymmetric cell division polarity, and neuronal axon proliferation during worm development [4 ] . mROR1 and mROR2 may play different roles in the development of the nervous system [5] . This product is ROR1-FC recombinant protein ( FC tag can be removed by enterokinase), which can be used for monoclonal antibody screening or antibody function verification.

Product name: Recombinant human source ROR1 protein

Species: Human origin

Gene sequence number: Q01973

Tags: Human-Fc Tag

Tag site: C terminal

Restriction site: enterokinase

Expression area: extracellular segment 30-403AA

Host cell:CHO

Purity: >90%

Purification method: Protein A column

Preservation system: PBS+20% glycerol

Storage conditions: -80℃

Diagram: Lane M on the left is the protein MW marker, Lane 1 is the 10ug ROR1-Fc protein;

The right picture shows the flow cytometric detection of the binding of ROR1-Fc protein to 293T overexpressing ROR1-CAR

Reference materials:

1. Expression of the Ror1 and Ror2 Receptor Tyrosine Kinase Genes During Mouse Development

2. Loss of mRor1 Enhances the Heart and Skeletal Abnormalities in mRor2-deficient Mice: Redundant and Pleiotropic Functions of mRor1 and mRor2 Receptor Tyrosine Kinases

3. Localization of Disulfide Bonds in the Frizzled Module of Ror1 Receptor Tyrosine Kinase

4. A C. Elegans Ror Receptor Tyrosine Kinase Regulates Cell Motility and Asymmetric Cell Division

5. Spatio-temporally Regulated Expression of Receptor Tyrosine Kinases, mRor1, mRor2, During Mouse Development: Implications in Development and Function of the Nervous System