Siglec molecules are a class of classic immunoglobulin-like lectins. At present, 15 human and 9 murine Siglec molecules have been found, which are usually expressed on cells of the innate immune system. The Siglec family belongs to type I membrane proteins with a single transmembrane. The N -terminus is composed of a V-set Ig domain that binds sialic acid and a certain number of C2-set Ig domains.
Siglec-10 is a member of the Siglec family. In the immune response, Siglec-10 has been shown to have a negative regulatory effect on the functions of the innate and acquired immune system. CD24, which is highly expressed in tumor cells, is one of the ligands of Siglecl 10 [1] . Studies have found that the combination of CD24 and Siglecl-10 triggers an inhibitory signal cascade. The intracellular tail contains immunoglobulin tyrosine. inhibitory motifs (ITIMs) of Siglecl-10 can be raised by tyrosine phosphatase, such as SH2 domain containing protein tyrosine phosphatases SHP-1 and SHP-2 , inhibitory signal transduction into the cell, thus blocking the TLR -mediated Inflammation and cytoskeletal rearrangement required by macrophages to engulf target cells [2] . By knocking out CD24 or Siglec-10 gene and blocking the interaction between CD24-Siglec-10 , the phagocytic ability of macrophages on tumor cells with high expression of CD24 can be improved , and the survival time of patients can be prolonged [3].
This product is Siglec-10 recombinant protein ( FC tag can be removed by enterokinase), which can be used for monoclonal antibody screening or antibody function verification.
Product name: Recombinant human source Siglec-10 protein
Species: Human origin
Gene sequence number: Q96LC7
Tags: Human-Fc Tag
Tag site: C terminal
Restriction site: enterokinase
Expression area: extracellular segment (17-546AA)
Host cell: CHO
Purity: ＞90%
Purification method: Protein A column
Preservation system: PBS+20% glycerol
Storage conditions: -80℃
Lane M on the left is the protein MW marker, Lane 1 is 15ug Siglec10(17-546)-Fc protein;
The picture on the right shows the flow cytometric detection of the binding of Siglec10-Fc protein to 293T overexpressing Siglec10-CAR
Reference materials:
1. The human B cell-associated antigen CD24 is a single chain sialoglycoprotein
2. CD24 and Siglec-10 Selectively Repress Tissue Damage–Induced Immune Responses
3. CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy
