The human EGFR protein is a transmembrane receptor, which can be used as a tyrosine kinase receptor to bind to homologous ligands, activate multiple signal levels and convert extracellular signals into appropriate cellular responses. The binding of its homologous ligands such as epidermal growth factor (EGF) and transforming growth factor alpha (TGFα) to the extracellular domain leads to dimerization of EGFR and autophosphorylation of tyrosine residues in the cytoplasmic domain ^[1] . Studies have shown that there are high or abnormal expressions of EGFR in many solid tumors . EGFR is related to the inhibition of tumor cell proliferation, angiogenesis, tumor invasion, metastasis, and apoptosis. Therefore , monoclonal antibodies targeting EGFR are often used in tumor targeted therapy ^[2,3] . Further, since EGFR do not express cell surface list T, in the framework of CAR-T when cells by T2A expression original total EGFR extracellular domain, the reaction may be indirectly CA expression in R.

This product is targeted EGFR monoclonal antibody (cetuximab) of the extracellular domain, can be used to EGFR overexpressing cell lines detected by flow cytometry, and EGFR activity in the recombinant protein.

Product name: EGFR monoclonal antibody

Reactivity: human

Subclass: human IgG1

Purification method: affinity chromatography

Storage system: PBS, pH 7.4, 20% glycerol

Storage conditions: -20°C refrigerator, avoid repeated freezing and thawing

Illustration : Lane M on the left is the protein MW marker, Lane 1 is the 4ug EGFR antibody;

The picture on the right shows the FACS detection of EGFR antibody binding to overexpressed EGFR CART.

Reference materials:

1. EGFR signaling pathway in breast cancers: from traditional signal transduction to direct nuclear translocalization

2.  Evaluation of the epidermal growth factor receptor gene mutation and copy number in non-small cell lung cancer with gefitinib therapy

3. Potential relationship between normalization of endometrial epidermal growth factor profile and restoration of fertility in repeat breeder cows