ABexpress Platform

In order to obtain diverse natural antibodies quickly and efficiently, iCarTab has developed the ABexpressR platform, which is a technology based on flow sorting, in vitro cloning and expression of antigen-specific B cell antibody genes-through different flow cytometry Combine antibodies and target antigens to sort out single B cells that have affinity for target antigens; clone a single B cell antibody-encoding gene by PCR technology and construct an expression vector. After transfecting the tool cell line, the cell culture supernatant can be verified Whether there is an antibody with affinity to the target antigen in the solution.

This method can avoid the many inconveniences caused by hybridoma and phage technology. The obtained antibody retains the natural pairing of the variable regions of the light and heavy chains. It has the characteristics of good gene diversity, high efficiency, short cycle, and suitable for any animal antibody screening. 


Hybridoma technology

Phage Technology

  • Cover all target-specific antibody secreting cells produced by immunized animals

  • Get accurate antibody gene coding sequence in one step in as short as six weeks

  • The platform can be extended to monoclonal antibodies or sdab of any animal

  • High throughput, 100+ different candidate antibodies can be obtained at one time

  • Hybridoma fusion efficiency is low, and many B cells cannot form stable fusion strains

  • A large number of cell cultures, the preparation cost is very high

  • Because of the limitation of myeloma cells, only murine monoclonal antibodies can be produced at present

  • Staff training time is long, workload is large, and throughput is low

  • Long monoclonal antibody preparation process (8-10 months) hybridoma cells are unstable and easily lead to the loss of antibody genes

  • During the phage construction process, there is no guarantee that the phage library will cover all sequences

  • The wrong structure may appear during the antibody display process, and the antibody cannot bind to the target antigen

  • Non-specific binding of phage to target cannot be avoided

  • The panning workload is huge, and the number of candidate antibodies obtained is small

At present, our company has used ABexpress R for a variety of antibody screening including single domain antibody and murine monoclonal antibodies, rabbit monoclonal antibodies, and fully human monoclonal antibodies.

The technical process for screening sdAb on ABexpress R platform is as follows: