In order to obtain diverse natural antibodies quickly and efficiently, iCarTab has developed the ABexpress^R platform, which is a technology based on flow sorting, in vitro cloning and expression of antigen-specific B cell antibody genes-through different flow cytometry Combine antibodies and target antigens to sort out single B cells that have affinity for target antigens; clone a single B cell antibody-encoding gene by PCR technology and construct an expression vector. After transfecting the tool cell line, the cell culture supernatant can be verified Whether there is an antibody with affinity to the target antigen in the solution.

This method can avoid the many inconveniences caused by hybridoma and phage technology. The obtained antibody retains the natural pairing of the variable regions of the light and heavy chains. It has the characteristics of good gene diversity, high efficiency, short cycle, and suitable for any animal antibody screening. 

At present, our company has used ABexpress R for a variety of antibody screening including single domain antibody and murine monoclonal antibodies, rabbit monoclonal antibodies, and fully human monoclonal antibodies.

The technical process for screening sdAb on ABexpress R platform is as follows: