Lentivirus Packaging Service
iCarTab adopts the third-generation recombinant lentivirus packaging system with the highest biological safety, and distributes Gag, Pol, Rev and VSV-G necessary for the preparation of recombinant lentivirus into different plasmids to maximize its biological safety. Preferred 293T cell clones and domesticated suspension 293TS cells can produce high-titer lentivirus in the shortest time. The serum-free lentivirus packaging system based on suspension cells can be used for clinical-grade lentivirus packaging services to meet the needs of clinical CAR-T lentivirus.
>>Service Features
The third-generation lentivirus packaging system further improves biosafety
TetOff-based inducible expression system produces high-titer lentivirus
Serum-free virus preparation system, completely get rid of the trouble of residual FBS
Preferred 293T clone, suspension and acclimation of 293TS cells, suitable for serum-free high-density culture
Tangential flow filtration to purify viruses, suitable for in vivo applications
Q-PCR determination of functional titer, bid farewell to the false high titer of ELISA
Provide clinical and scientific CAR-T virus packaging services
>>Serum-free culture system
>>Technical Process
>>Case Show
Illustration: Flow cytometry and fluorescence microscopy to detect the efficiency of GFP lentivirus transfection into natural T cells
Lentiviral vector preparation
Transient transfection of packaging cells
Virus supernatant collection
Lentivirus concentration and titer determination
Delivery within 2-4 weeks
Target gene ID (CDS sequence NM_XXXXX.X) or target gene ORF sequence information
5μg of plasmid containing the target gene and plasmid details
Required virus titer and purification level
Cloning vector containing the target gene 4μg
Recombinant Lentivirus
experimental report
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